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KMID : 0613820070170091260
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Journal of Life Science
2007 Volume.17 No. 9 p.1260 ~ p.1265
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Development of α-Amylase Coated Magnetic Nanofiber for the Hydrolysis of Starch
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Lee Jung-Heon
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Abstract
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Magnetically separable enzyme-coated nanofibers were developed for the hydrolysis of starch. Stability of α-amylase-coated nanofiber was greatly improved and its residual activity was maintained over 92.7% after 32 days incubation at room temperature and under shaking conditions (200 rpm). The recovery of enzyme was high and enzyme activity after 10 recycle was 95.2% of its original activity. Developed enzyme-coated nanofibers were used for the hydrolysis of starch. When 0.5 mg of magnetically separable enzyme nanofibers was used, 40 g/l of starch (2 ml) was completely degraded within 40 min. The continuous enzyme reactor was developed and used for starch hydrolysis and 76% of starch (30 g/l) was hydrolyzed with 1 hr residence time.
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KEYWORD
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Nanoenzyme, amylase, starch, magnetic nanofiber
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